The very existence of the travels of Hasekura was forgotten in Japan until the reopening of the country after the Sakoku policy of isolation. In 1873, a Japanese embassy to Europe (the Iwakura mission) headed by Iwakura Tomomi heard for the first time of the travels of Hasekura when shown documents during their visit to Venice in Italy.
Today, there are statues of Hasekura Tsunenaga in the outskirts of Acapulco in Mexico, at the entrance of Havana Bay in Cuba, in Coria del Río in Spain, in the Church of Civitavecchia in Italy, and in Tsukinoura, near Ishinomaki.
Approximately 700 inhabitants of Coria del Río bear the surname Japón (originally Hasekura de Japón), identifying them as descendants of the members of Hasekura Tsunenaga's delegation.
A theme park describing the embassy and displaying a replica of the San Juan Bautista was established in the harbour of Ishinomaki, from which Hasekura initially departed on his voyage.
Today there stands a statue of Hasekura in a park in Manila, the Philippines.
Endo Shusaku wrote a 1980 novel, titled The Samurai, a fictious account relating the travels of Hasekura.
A 2005 animation film produced in Spain and titled Gisaku relates the adventures of a young Japanese samurai named Yohei who visited Spain in the 17th century, in a story loosely taking its inspiration from the travels of Hasekura. Yohei survived in hiding to the present day due to magical powers ("After centuries of lethargy, he awakes in a World he does not know"), and accomplishes many adventures in modern Europe as a superhero.